Mitochondria and sarcoplasmic reticulum as model targets for neurotoxic and myotoxic phospholipases A2
نویسندگان
چکیده
منابع مشابه
Prediction of Myotoxic and Neurotoxic Activities in Phospholipases A2 from Primary Sequence Analysis
We developed a methodology to predict myotoxicity and neurotoxicity of proteins of the family of Phospholipases A2 (PLA2) from sequence data. Combining two bioinformatics tools, MEME and HMMER, it was possible to detect conserved motifs and represent them as Hidden Markov Models (HMMs). In ten-fold cross validation testing we have determined the efficacy of each motif on prediction of PLA2 func...
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Muscle cells, and in particular cardiomyocytes, consume a large amount of chemical energy to produce mechanical work. Myofilament contraction is regulated and synchronized within the cell by rapid, transient changes in the cytosolic Ca2þ concentration. Most of this calcium is released from the sarcoplasmic reticulum (SR), which has a large surface for Ca2þ transport, in close contact with the m...
متن کاملCrystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A2 from Bothrops brazili venom.
Two myotoxic and noncatalytic Lys49-phospholipases A(2) (braziliantoxin-II and MT-II) and a myotoxic and catalytic phospholipase A(2) (braziliantoxin-III) from the venom of the Amazonian snake Bothrops brazili were crystallized. The crystals diffracted to resolutions in the range 2.56-2.05 Å and belonged to space groups P3(1)21 (braziliantoxin-II), P6(5)22 (braziliantoxin-III) and P2(1) (MT-II)...
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WOOD, AND ARNOLD SCHWARTZ. Mitochondria and sarcoplasmic reticulum function in cardA hypertrophy and failure. Am. J. Physiol. 224(3) : 497-502. 1973.-Mitochondria and “sarcoplasmic reticulum” cardiac relaxing system (CRS) were isolated from rabbit hearts that were in various stages of hypertrophy and failure induced by progressive stenosis of the ascending aorta. The latter was produced by Amer...
متن کاملNeurotoxic phospholipase A2 toxicity model
The molecular mechanism of action of presynaptically neurotoxic secreted phospholipases A2 (sPLA2s) has not been fully elucidated. We have recently proposed a model to explain one of the hallmarks of their action - the reduction in endocytosis leading to synaptic vesicle depletion in nerve terminals. Our results speak strongly in favor of a mechanism in which both specific protein-protein inter...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.3.1346